Journal: The Journal of pediatrics

Article Title: Using Multiplex Molecular Testing to Determine the Etiology of Acute Gastroenteritis in Children

doi: 10.1016/j.jpeds.2016.05.068

Figure Lengend Snippet: Determination of positive specimens

Article Snippet: 13 Gastrointestinal pathogen targets table ft1 table-wrap mode="anchored" t5 caption a7 Assay Pathogen Determination of specimen positivity GPP * performed at Vanderbilt with or without GPP and/or single-plex PCR performed at the Luminex Molecular Diagnostics Laboratory Campylobacter spp Screen-positive at Vanderbilt using GPP and subsequently confirmed at the Luminex Molecular Diagnostics Laboratory using single-plex and/or multiplex PCR with primers specific for pathogenic Campylobacter species ( jejuni , coli , and lari ) * C difficile Codetection of toxin A and toxin B or reproducible detection of either toxin A or toxin B Enterotoxigenic E coli (heat-labile and heat-stable toxins) Median fluorescence intensity ≥2 times the target-specific positivity threshold E coli O157 Salmonella spp Shiga toxin-producing E coli (Shiga toxins 1 and 2) Vibrio cholerae Shigella spp Yersinia enterocolitica Adenovirus serotypes 40 and 41 Norovirus GI Norovirus GII Rotavirus group A Cryptosporidium spp Entamoeba histolytica Giardia lamblia Duplex RT-rtPCR assay performed at Vanderbilt Astrovirus Target-specific Ct value ≤35 Sapovirus Open in a separate window GI , Genogroup I.

Techniques: Luminex, Multiplex Assay, Fluorescence